LOCUS       40924_320       12525 bp DNA     circular SYN 17-DEC-2018
DEFINITION  BAC cloning vector attB-P[acman]-CmR-F-2-attB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12525)
  AUTHORS   Venken KJ, He Y, Hoskins RA, Bellen HJ.
  TITLE     P[acman]: a BAC transgenic platform for targeted insertion of large 
            DNA fragments in D. melanogaster
  JOURNAL   Science 314 (5806), 1747-1751 (2006)
  PUBMED    17138868
REFERENCE   2  (bases 1 to 12525)
  AUTHORS   Venken KJT., He Y, Hoskins RA, Bellen HJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2006) Molecular and Human Genetics, Baylor College
            of Medicine, One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 12525)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12525)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2006"; volume: "314"; issue: "5806"; pages: "1747-1751"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-NOV-2006) Molecular and Human Genetics, Baylor College of 
            Medicine, One Baylor Plaza, Houston, TX 77030, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(244..717)
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(793..1761)
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(1764..2936)
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    complement(3262..3512)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3518..4270)
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      complement(4361..4580)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      4659..5372
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6236..6338
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             6339..6995
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     protein_bind    7134..7203
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_feature    complement(7418..7650)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     7657..7673
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7683..7701
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7745..7819
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(7838..7856)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    7892..11907
                     /label=white dominant eye marker
                     /note="white dominant eye marker"
     CDS             join(8376..8447,8849..9122,9197..9851,9913..10228,
                     10432..10563,10634..11248)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    complement(11908..12493)
                     /label=P element 5' end
ORIGIN
        1 tcgacagcga cacacttgca tcggatgcag cccggttaac gtgccggcac ggcctgggta
       61 accaggtatt ttgtccacat aaccgtgcgc aaaatgttgt ggataagcag gacacagcag
      121 caatccacag caggcataca accgcacacc gaggttactc cgttctacag gttacgacga
      181 catgtcaata cttgcccttg acaggcattg atggaatcgt agtctcacgc tgatagtctg
      241 atcgacaata caagtgggac cgtggtccca gaccgataat cagaccgaca acacgagtgg
      301 gatcgtggtc ccagactaat aatcagaccg acgatacgag tgggaccgtg gtcccagact
      361 aataatcaga ccgacgatac gagtgggacc gtggttccag actaataatc agaccgacga
      421 tacgagtggg accgtggtcc cagactaata atcagaccga cgatacgagt gggaccatgg
      481 tcccagacta ataatcagac cgacgatacg agtgggaccg tggtcccagt ctgattatca
      541 gaccgacgat acgagtggga ccgtggtccc agactaataa tcagaccgac gatacgagtg
      601 ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt ggtcccagtc
      661 tgattatcag accgacgata caagtggaac agtgggccca gagagaatat tcaggccagt
      721 tatgctttct ggcctgtaac aaaggacatt aagtaaagac agataaacgt agactaaaac
      781 gtggtcgcat cagggtgctg gcttttcaag ttccttaaga atggcctcaa ttttctctat
      841 acactcagtt ggaacacgag acctgtccag gttaagcacc attttatcgc ccttatacaa
      901 tactgtcgct ccaggagcaa actgatgtcg tgagcttaaa ctagttcttg atgcagatga
      961 cgttttaagc acagaagtta aaagagtgat aacttcttca gcttcaaata tcaccccagc
     1021 ttttttctgc tcatgaaggt tagatgcctg ctgcttaagt aattcctctt tatctgtaaa
     1081 ggctttttga agtgcatcac ctgaccgggc agatagttca ccggggtgag aaaaaagagc
     1141 aacaactgat ttaggcaatt tggcggtgtt gatacagcgg gtaataatct tacgtgaaat
     1201 attttccgca tcagccagcg cagaaatatt tccagcaaat tcattctgca atcggcttgc
     1261 ataacgctga ccacgttcat aagcacttgt tgggcgataa tcgttaccca atctggataa
     1321 tgcagccatc tgctcatcat ccagctcgcc aaccagaaca cgataatcac tttcggtaag
     1381 tgcagcagct ttacgacggc gactcccatc ggcaatttct atgacaccag atactcttcg
     1441 accgaacgcc ggtgtctgtt gaccagtcag tagaaaagaa gggatgagat catccagtgc
     1501 gtcctcagta agcagctcct ggtcacgttc attacctgac catacccgag aggtcttctc
     1561 aacactatca ccccggagca cttcaagagt aaacttcaca tcccgaccac atacaggcaa
     1621 agtaatggca ttaccgcgag ccattactcc tacgcgcgca attaacgaat ccaccatcgg
     1681 ggcagctggt gtcgataacg aagtatcttc aaccggttga gtattgagcg tatgttttgg
     1741 aataacaggc gcacgcttca ttatctaatc tcccagcgtg gtttaatcag acgatcgaaa
     1801 atttcattgc agacaggttc ccaaatagaa agagcatttc tccaggcacc agttgaagag
     1861 cgttgatcaa tggcctgttc aaaaacagtt ctcatccgga tctgaccttt accaacttca
     1921 tccgtttcac gtacaacatt ttttagaacc atgcttcccc aggcatcccg aatttgctcc
     1981 tccatccacg gggactgaga gccattacta ttgctgtatt tggtaagcaa aatacgtaca
     2041 tcaggctcga accctttaag atcaacgttc ttgagcagat cacgaagcat atcgaaaaac
     2101 tgcagtgcgg aggtgtagtc aaacaactca gcaggcgtgg gaacaatcag cacatcagca
     2161 gcacatacga cattaatcgt gccgataccc aggttaggcg cgctgtcaat aactatgaca
     2221 tcatagtcat gagcaacagt ttcaatggcc agtcggagca tcaggtgtgg atcggtgggc
     2281 agtttacctt catcaaattt gcccattaac tcagtttcaa tacggtgcag agccagacag
     2341 gaaggaataa tgtcaagccc cggccagcaa gtgggcttta ttgcataagt gacatcgtcc
     2401 ttttccccaa gatagaaagg caggagagtg tcttctgcat gaatatgaag atctggtacc
     2461 catccgtgat acattgaggc tgttccctgg gggtcgttac cttccacgag caaaacacgt
     2521 agccccttca gagccagatc ctgagcaaga tgaacagaaa ctgaggtttt gtaaacgcca
     2581 cctttatggg cagcaacccc gatcaccggt ggaaatacgt cttcagcacg tcgcaatcgc
     2641 gtaccaaaca catcacgcat atgattaatt tgttcaattg tataaccaac acgttgctca
     2701 acccgtcctc gaatttccat atccgggtgc ggtagtcgcc ctgctttctc ggcatctctg
     2761 atagcctgag aagaaacccc aactaaatcc gctgcttcac ctattctcca gcgccgggtt
     2821 attttcctcg cttccgggct gtcatcatta aactgtgcaa tggcgatagc cttcgtcatt
     2881 tcatgaccag cgtttatgca ctggttaagt gtttccatga gtttcattct gaacatcctt
     2941 taatcattgc tttgcgtttt tttattaaat cttgcaattt actgcaaagc aacaacaaaa
     3001 tcgcaaagtc atcaaaaaac cgcaaagttg tttaaaataa gagcaacact acaaaaggag
     3061 ataagaagag cacatacctc agtcacttat tatcactagc gctcgccgca gccgtgtaac
     3121 cgagcatagc gagcgaactg gcgaggaagc aaagaagaac tgttctgtca gatagctctt
     3181 acgctcagcg caagaagaaa tatccaccgt gggaaaaact ccaggtagag gtacacacgc
     3241 ggatagccaa ttcagagtaa taaactgtga taatcaaccc tcatcaatga tgacgaacta
     3301 acccccgata tcaggtcaca tgacgaaggg aaagagaagg aaatcaactg tgacaaactg
     3361 ccctcaaatt tggcttcctt aaaaattaca gttcaaaaag tatgagaaaa tccatgcagg
     3421 ctgaaggaaa cagcaaaact gtgacaaatt accctcagta ggtcagaaca aatgtgacga
     3481 accaccctca aatctgtgac agataaccct cagactatcc tgtcgtcatg gaagtgatat
     3541 cgcggaagga aaatacgata tgagtcgtct ggcggccttt ctttttctca atgtatgaga
     3601 ggcgcattgg agttctgctg ttgatctcat taacacagac ctgcaggaag cggcggcgga
     3661 agtcaggcat acgctggtaa ctttgaggca gctggtaacg ctctatgatc cagtcgattt
     3721 tcagagagac gatgcctgag ccatccggct tacgatactg acacagggat tcgtataaac
     3781 gcatggcata cggattggtg atttcttttg tttcactaag ccgaaactgc gtaaaccggt
     3841 tctgtaaccc gataaagaag ggaatgagat atgggttgat atgtacactg taaagccctc
     3901 tggatggact gtgcgcacgt ttgataaacc aaggaaaaga ttcatagcct ttttcatcgc
     3961 cggcatcctc ttcagggcga taaaaaacca cttccttccc cgcgaaactc ttcaatgcct
     4021 gccgtatatc cttactggct tccgcagagg tcaatccgaa tatttcagca tatttagcaa
     4081 catggatctc gcagataccg tcatgttcct gtagggtgcc atcagatttt ctgatctggt
     4141 caacgaacag atacagcata cgtttttgat cccgggagag actatatgcc gcctcagtga
     4201 ggtcgtttga ctggacgatt cgcgggctat ttttacgttt cttgtgattg ataaccgctg
     4261 tttccgccat gacagatcca tgtgaagtgt gacaagtttt tagattgtca cactaaataa
     4321 aaaagagtca ataagcaggg ataactttgt gaaaaaacag cttcttctga gggcaatttg
     4381 tcacagggtt aagggcaatt tgtcacagac aggactgtca tttgagggtg atttgtcaca
     4441 ctgaaagggc aatttgtcac aacaccttct ctagaaccag catggataaa ggcctacaag
     4501 gcgctctaaa aaagaagatc taaaaactat aaaaaaaata attataaaaa tatccccgtg
     4561 gataagtgga taaccccaag ggaagttttt tcaggcatcg tgtgtaagca gaatatataa
     4621 gtgctgttcc ctggtgcttc ctcgctcact cgaaattccc ctttccgacg ctcaccgggc
     4681 tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc
     4741 cgtcgaagcc gtgtgcgaga caccgcggcc ggccgccggc gttgtggata cctcgcggaa
     4801 aacttggccc tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc
     4861 ggcgcggcgt tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca
     4921 gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca
     4981 agcctgggga taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga
     5041 ggggcgcgat ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg
     5101 acatttgagg ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt
     5161 ttcggccacc gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt
     5221 tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct
     5281 tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc
     5341 ggccgcgaac ggcctcaccc caaaaatggc aggggatctc gagggcttcg ccctgtcgct
     5401 cgactgcggc gagcactact ggctgtaaaa ggacagacca catcatggtt ctgtgttcat
     5461 taggttgttc tgtccattgc tgacataatc cgctccactt caacgtaaca ccgcacgaag
     5521 atttctattg ttcctgaagg catattcaaa tcgttttcgt taccgcttgc aggcatcatg
     5581 acagaacact acttcctata aacgctacac aggctcctga gattaataat gcggatctct
     5641 acgataatgg gagattttcc cgactgtttc gttcgcttct cagtggataa cagccagctt
     5701 ctctgtttaa cagacaaaaa cagcatatcc actcagttcc acatttccat ataaaggcca
     5761 aggcatttat tctcaggata attgtttcag catcgcaacc gcatcagact ccggcatcgc
     5821 aaactgcacc cggtgccggg cagccacatc cagcgcaaaa accttcgtgt agacttccgt
     5881 tgaactgatg gacttatgtc ccatcaggct ttgcagaact ttcagcggta taccggcata
     5941 cagcatgtgc atcgcatagg aatggcggaa cgtatgtggt gtgaccggaa cagagaacgt
     6001 cacaccgtca gcagcagcgg cggcaaccgc ctccccaatc caggtcctga ccgttctgtc
     6061 cgtcacttcc cagatccgcg ctttctctgt ccttcctgtg cgacggttac gccgctccat
     6121 gagcttatcg cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg
     6181 gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc
     6241 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
     6301 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     6361 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     6421 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     6481 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     6541 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     6601 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     6661 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     6721 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     6781 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     6841 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     6901 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     6961 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt
     7021 gcccttaaac gcctggttgc tacgcctgaa taagtgataa taagcggatg aatggcagaa
     7081 attcgatgat aagctgtcaa acatgagaat tggtcgacga tgtaggtcac ggtctcgaag
     7141 ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc cacctcaccc
     7201 atctggtcca tcatgatgaa cgggtcgagg tggcggtagt tgatcccggc gaacgcgcgg
     7261 cgcaccggga agccctcgcc ctcgaaaccg ctgggcgcgg tggtcacggt gagcacggga
     7321 cgtgcgacgg cgtcggcggg tgcggatacg cggggcagcg tcagcgggtt ctcgacggtc
     7381 acggcgggca tgtcgatcac gatgagaatg gccagaccat gatgaaataa cataaggtgg
     7441 tcccgtcggc aagagacatc cacttaacgt atgcttgcaa taagtgcgag tgaaaggaat
     7501 agtattctga gtgtcgtatt gagtctgagt gagacagcga tatgattgtt gattaaccct
     7561 tagcatgtcc gtggggtttg aattaactca taatattaat tagacgaaat tatttttaaa
     7621 gttttatttt taataatttg cgagtacgca accggtgtaa aacgacggcc agtgagcgcg
     7681 cgtaatacga ctcactatag ggcgaattgg agctcgttta aacctcgagc ggtccgttat
     7741 cgatggcgcg ccggccttaa tggccgcggc cgcatttaaa tgtcgactta attaaggccg
     7801 gccgcgatcg cgcccgggcg gtacccagct tttgttccct ttagtgaggg ttaattgcgc
     7861 gcttggcgta atcatggtca tagctgtttc cgaattctag tatgtatgta agttaataaa
     7921 acccattttt gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc
     7981 attgaactta tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg
     8041 ttctttcgat tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc
     8101 cttctctgtc cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc
     8161 caccgtttgt agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt
     8221 tcgtgacgaa gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac
     8281 tcctctcgct tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg
     8341 tgggtaagca ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat
     8401 taattcgcgg aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat
     8461 gtgcatacat cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtct
     8521 gcggcgtgag aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga
     8581 cacgaaacag attattctgg tagctgttct cgctatataa gacaattttt gagatcatat
     8641 catgatcaag acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata
     8701 acaaccagat attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta
     8761 cttcgtagga tacttcgggg tactttttgt tcggggttag atgagcataa cgcttgtagt
     8821 tgatatttga gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat
     8881 taaccagggc ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga
     8941 ggactccggt tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat
     9001 ggacatcttt ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac
     9061 acgcggacta ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa
     9121 cggtgagttt ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta
     9181 ccaatttgaa actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc
     9241 agttccggtg ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc
     9301 atccaagtat cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag
     9361 atgcaggcca ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc
     9421 agggaacacc tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag
     9481 cgagtggccc gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg
     9541 atcatcggtg tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca
     9601 ttcgcctccg aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga
     9661 ctggactcct ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc
     9721 aagaccgtca tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag
     9781 atccttctga tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac
     9841 ttcttttcct agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca
     9901 actcctatcc agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt
     9961 acaggtgttg gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat
    10021 atgcgacaat tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa
    10081 aaatctggag aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt
    10141 catgcagttc cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct
    10201 cgtaaaagtg cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa
    10261 cgcttacaat tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac
    10321 ccttcttagt ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt
    10381 tcatttaact cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc
    10441 atcttgattg gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc
    10501 aacggagcca tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata
    10561 aatgtaagtc atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat
    10621 cgattcgatt taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg
    10681 actttatcgc tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct
    10741 cacagtgcca ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt
    10801 gctgcacttc ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt
    10861 cggatatcta atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc
    10921 ggttatcata ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt
    10981 atacctcaaa tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat
    11041 taaccaatgg gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg
    11101 ccccagttcg ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct
    11161 ggactacgtg ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct
    11221 aagacttcgg gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt
    11281 tttttttacc attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta
    11341 attatatttg tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg
    11401 aacatcccca caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat
    11461 accttacatc aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac
    11521 atacttttca aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt
    11581 tgctctttcg ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct
    11641 tggagcgact gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca
    11701 tttcgtctca atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat
    11761 atttgtatat atttccctca cagctatatt tattctaatt taatattatg actttttaag
    11821 gtaatttttt gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc
    11881 cagtgtttgt tccttgtgta gatgcatgct gctgctctaa acgacgcatt tcgtactcca
    11941 aagtacgaat tttttccctc aagctcttat tttcattaaa caatgaacag gacctaacgc
    12001 acagtcacgt tattgtttac ataaatgatt ttttttacta ttcaaactta ctctgtttgt
    12061 gtactcccac tggtatagcc ttcttttatc ttttctggtt caggctctat cactttacta
    12121 ggtacggcat ctgcgttgag tcgcctcctt ttaaatgtct gaccttttgc aggtgcagcc
    12181 ttccactgcg aatcattaaa gtgggtatca caaatttggg agttttcacc aaggctgcac
    12241 ccaaggctct gctcccacaa ttttctctta atagcacact tcggcacgtg aattaatttt
    12301 actccagtca cagctttgca gcaaaatttg caatatttca ttttttttta ttccacgtaa
    12361 gggttaatgt tttcaaaaaa aaattcgtcc gcacacaacc tttcctctca acaagcaaac
    12421 gtgcactgaa tttaagtgta tacttcggta agcttcggct atcgacggga ccaccttatg
    12481 ttatttcatc atgggccaga cccacgtagt ccagactagt ggatc
//
